LIGAND-BINDING KINETICS OF A SOLUBLE FULL-LENGTH MURINE ERYTHROPOIETIN RECEPTOR

被引：4

作者：

AVEDISSIAN, LS ^{[1
]}

POOLA, I ^{[1
]}

SPIVAK, JL ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DIV HEMATOL,BALTIMORE,MD 21205

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 216卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.2592

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The full-length murine erythropoietin receptor was expressed in Sf9 cells using a baculovirus vector. Erythropoietin receptors in solubilized Sf9 cell lysates bound erythropoietin with high affinity (92 pM) Erythropoietin receptor-I-125-labeled erythropoietin association and dissociation kinetics using solubilized Sf9 cell lysates revealed a ka of 0.16 nM(-2) min(-1) and a kd of 0.00055 min(-1) giving an observed KD of 3.45 pM. The erythropoietin receptor was partially purified from Sf9 cell lysates by chromatography on Con A Sepharose. When erythropoietin receptors were crosslinked to I-125-labeled erythropoietin and analyzed by SDS-7.5% PAGE protein complexes of 90 and 125 kDa were observed with receptors in solubilized lysate, and 170 and 190 kDa with the partially purified receptors. (C) 1995 Academic Press, Inc.

引用

页码：62 / 68

页数：7

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