UNDERSTANDING AND CONTROLLING TRANSGENE EXPRESSION

被引：118

作者：

MEYER, P

机构：

[1] The Centre for Plant Biochemistry and Biotechnology, Department of Genetics, University of Leeds, Leeds

来源：

TRENDS IN BIOTECHNOLOGY | 1995年 / 13卷 / 09期

关键词：

D O I：

10.1016/S0167-7799(00)88977-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Instability of transgene expression is frequently observed in transgenic plants, and is commonly called 'gene silencing', However, silencing that occurs at the transcriptional or post-transcriptional level is not limited to transgenes; endogenous genes can also be suppressed after the transfer of a homologous transgene, Inactivation of transgene expression is often accompanied by an increase in DNA methylation, especially if silencing occurs at the transcriptional level. A comparative study of different silencing systems should help to define the critical requirements for the reliable expression of recombinant constructs in transgenic plants.

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页码：332 / 337

页数：6

相关论文

共 40 条

[1] CHANGES IN T-DNA METHYLATION AND EXPRESSION ARE ASSOCIATED WITH PHENOTYPIC VARIATION AND PLANT-REGENERATION IN A CROWN GALL TUMOR LINE [J].

AMASINO, RM ;

POWELL, ALT ;

GORDON, MP .

MOLECULAR & GENERAL GENETICS, 1984, 197 (03) :437-446

[2] EPIGENETIC REPEAT-INDUCED GENE SILENCING (RIGS) IN ARABIDOPSIS [J].

ASSAAD, FF ;

TUCKER, KL ;

SIGNER, ER .

PLANT MOLECULAR BIOLOGY, 1993, 22 (06) :1067-1085

[3] DNA METHYLATION - EVOLUTION OF A BACTERIAL IMMUNE FUNCTION INTO A REGULATOR OF GENE-EXPRESSION AND GENOME STRUCTURE IN HIGHER EUKARYOTES [J].

BESTOR, TH .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1990, 326 (1235) :179-187

[4] CHARACTERIZATION OF A PLANT SCAFFOLD ATTACHMENT REGION IN A DNA FRAGMENT THAT NORMALIZES TRANSGENE EXPRESSION IN TOBACCO [J].

BREYNE, P ;

VANMONTAGU, M ;

DEPICKER, A ;

GHEYSEN, G .

PLANT CELL, 1992, 4 (04) :463-471

[5] TRANSGENE INACTIVATION - PLANTS FIGHT BACK [J].

FINNEGAN, J ;

MCELROY, D .

BIO-TECHNOLOGY, 1994, 12 (09) :883-888

[6] INACTIVATION OF GENE-EXPRESSION IN PLANTS AS A CONSEQUENCE OF SPECIFIC SEQUENCE DUPLICATION [J].

FLAVELL, RB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (09) :3490-3496

[7] RNA-DEPENDENT RNA-POLYMERASES OF PLANTS [J].

FRAENKELCONRAT, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (02) :422-424

[8] TARGETED TRANSFORMATION OF ASCOBOLUS-IMMERSUS AND DENOVO METHYLATION OF THE RESULTING DUPLICATED DNA-SEQUENCES [J].

GOYON, C ;

FAUGERON, G .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (07) :2818-2827

[9] DOES CO-SUPPRESSION OF SENSE GENES IN TRANSGENIC PLANTS INVOLVE ANTISENSE RNA [J].

GRIERSON, D ;

FRAY, RG ;

HAMILTON, AJ ;

SMITH, CJS ;

WATSON, CF .

TRENDS IN BIOTECHNOLOGY, 1991, 9 (04) :122-123

[10] POSITION-EFFECT VARIEGATION AFTER 60 YEARS [J].

HENIKOFF, S .

TRENDS IN GENETICS, 1990, 6 (12) :422-426

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