PREDICTION OF THE ELECTROPHORETIC MOBILITIES OF NUCLEOTIDES ON NEUTRAL PAPER

被引：6

作者：

SOMMER, SS

机构：

[1] The Rockefeller University, New York, NY 10021

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90698-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The electrophoretic mobilities of nucleotides on neutral paper can be predicted by U = kQ M 2 3, where U = electrophoretic mobility, k = proportionality constant, Q = charge, and M = molecular weight. The equation is accurate for molecules with molecular weights in the range 200-2000. The equation is useful for predicting whether a given set of nucleotides can be adequately separated at a particular pH. It is also valuable in the initial identification of unknown nucleotides. © 1979.

引用

页码：8 / 12

页数：5