FORMATION AND PERSISTENCE OF DNA ADDUCTS IN THE LIVER OF BROWN BULLHEADS EXPOSED TO BENZO[A]PYRENE

被引：56

作者：

SIKKA, HC ^{[1
]}

RUTKOWSKI, JP ^{[1
]}

KANDASWAMI, C ^{[1
]}

KUMAR, S ^{[1
]}

EARLEY, K ^{[1
]}

GUPTA, RC ^{[1
]}

机构：

[1] BAYLOR UNIV,DEPT PHARMACOL,HOUSTON,TX 77030

来源：

CANCER LETTERS | 1990年 / 49卷 / 01期

关键词：

anti-benzo[a]pyrene-7,8-diol-9, 10-epoxide-deoxyguanosine; benzo[a]pyrene; DNA adduct formation and persistence; Ictalurus nebulosus; metabolic activation;

D O I：

10.1016/0304-3835(90)90142-K

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The formation and persistence of benzo[a]pyrene (BP)-DNA adducts in the liver of brown bullheads (Ictalurus nebulosus) treated with the hydrocarbon (20 mg/kg body wt, i.p.) was investigated using the 32P-postlabeling assay. The highest level of covalent binding of BP to liver DNA (188 fmol BP adducts/mg DNA) was observed 25-30 days following treatment. After 70 days, the adduct level in liver DNA had declined to approximately 26% of the maximum adduct level. One major BP-DNA adduct and several minor ones were detected in the liver. The major adduct co-chromatographed with anti-BP-7,8-diol-9,10-epoxide-deoxyguanosine (anti-BPDE-dGuo) adduct. The data suggest that brown bullheads metabolically activate BP by the same mechanism as the mammalian systems susceptible to carcinogenic effects of the hydrocarbon. © 1990.

引用

页码：81 / 87

页数：7

共 34 条

[1] ASHURST SW, 1983, CANCER RES, V43, P1024
[2] BAUMANN PC, 1982, POLYNUCLEAR AROMATIC, P93
[3] BLACK J, 1985, WATER CHLORINATION C, V5, P415
[4] FIELD AND LABORATORY STUDIES OF ENVIRONMENTAL CARCINOGENESIS IN NIAGARA RIVER FISH
BLACK, JJ
[J]. JOURNAL OF GREAT LAKES RESEARCH, 1983, 9 (02) : 326 - 334
[5] BOROUJERDI M, 1981, CANCER RES, V41, P951
[6] BINDING TO MOUSE SKIN DNA OF BENZO[A]PYRENE, ITS 7,8-DIOL AND 7,8-DIOL-9,10-EPOXIDES IN RELATION TO THE TUMORIGENICITY OF THESE COMPOUNDS
BROOKES, P
[J]. CANCER LETTERS, 1979, 6 (4-5) : 285 - 289
[7] CONNEY AH, 1982, CANCER RES, V42, P4875
[8] COOPER CS, 1983, PROG DRUG METAB, V7, P295
[9] DUNN BP, 1987, CANCER RES, V47, P6543
[10] GLATT H, 1908, CARCINOGENESIS, V8, P1621

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