QUANTITATIVE-ANALYSIS OF ERYTHROMYCIN BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY USING COLUMN-SWITCHING

被引:21
作者
CACHET, T [1 ]
DETURCK, K [1 ]
ROETS, E [1 ]
HOOGMARTENS, J [1 ]
机构
[1] CATHOLIC UNIV LEUVEN,INST FARMACEUT WETENSCHAPPEN,FARMACEUT CHEM LAB,VAN EVENSTR 4,B-3000 LOUVAIN,BELGIUM
关键词
REVERSED-PHASE LIQUID CHROMATOGRAPHY; COLUMN-SWITCHING; ERYTHROMYCIN;
D O I
10.1016/0731-7085(91)80176-A
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A column-switching technique is described for LC of erythromycin. The method allows, in about 1 h, the separation of erythromycin A from all its known potential impurities, except erythromycin D, which is a minor impurity. The switching technique combines two columns (7.5 cm x 4.6 mm and 25.0 cm x 4.6 mm) both packed with RSil C 18 LL 10-mu-m. The mobile phase is acetonitrile-tetrabutylammonium sulphate (0.2 M, pH 6.0)-ammonium phosphate buffer (0.2 M, pH 6.0)-water (24:5:5:66, v/v/v/v). Temperature was 35-degrees-C, flow rate was 1.5 ml min-1, detection was by UV at 210 nm. Results for a number of commercial samples of various origin are reported.
引用
收藏
页码:547 / 555
页数:9
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