PKC-EPSILON IS INVOLVED IN GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR SIGNAL TRANSDUCTION - EVIDENCE FROM MICROPHYSIOMETRY AND ANTISENSE OLIGONUCLEOTIDE EXPERIMENTS

We have used microphysiometry and antisense methodology to show that the e isoenzyme of protein kinase C (PKC) is involved in the signal transduction pathway of granulocyte-macrophage colony-stimulating factor (GM-CSF) in a human bone marrow cell line, TF-1. These cells require GM-CSF or a related cytokine for proliferation. When the cells are appropriately exposed to GM-CSF, they exhibit a burst of metabolic activity that can be detected on the time scale of minutes in the microphysiometer, a biosensor-based instrument that measures the rate at which cells excrete protons. These cells express PKCalpha and -epsilon, as determined by Western blot analysis. Treatment with isoenzyme-specific antisense oligonucleotides inhibits expression appropriately, but only inhibition of PKCepsilon appreciably diminishes the burst of metabolic activity induced by GM-CSF. Consistent with the involvement of PKCepsilon, GM-CSF appears to activate phospholipase D and does not cause a detectable increase in cytosolic [Ca2+].