PURIFICATION OF THE PRO-PHENOL OXIDASE ENZYME FROM HEMOCYTES OF THE COCKROACH BLABERUS-DISCOIDALIS

被引：52

作者：

DURRANT, HJ

RATCLIFFE, NA

HIPKIN, CR

ASPAN, A

SODERHALL, K

机构：

[1] UNIV COLL SWANSEA,SCH BIOL SCI,SWANSEA SA2 8PP,W GLAM,WALES

[2] UNIV UPPSALA,DEPT PHYSIOL BOT,S-75121 UPPSALA,SWEDEN

来源：

BIOCHEMICAL JOURNAL | 1993年 / 289卷

关键词：

D O I：

10.1042/bj2890087

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pro-phenol oxidase was purified from the haemocytes of the cockroach Blaberus discoidalis by Blue Sepharose chromatography, hydrophobic-interaction chromatography on a Phenyl-Superose column and, finally, gel filtration on a Superose 6 column. Results suggest that the molecule exists as a polymer of identical 76 kDa monomeric units. The enzyme is a glycoprotein with pl of 5.2 and can be converted by trypsin into phenol oxidase.

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页码：87 / 91

页数：5

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