THE STEREOCHEMICAL COURSE OF GROUP-II INTRON SELF-SPLICING

被引：90

作者：

PADGETT, RA

PODAR, M

BOULANGER, SC

PERLMAN, PS

机构：

[1] Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235

来源：

SCIENCE | 1994年 / 266卷 / 5191期

关键词：

D O I：

10.1126/science.7527587

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The stereochemical specificities and reaction courses for both self-splicing steps of a group II intron have been determined by phosphorothioate substitution at the 5' and 3' splice site phosphodiester bonds. Both steps of the splicing reaction proceeded with a phosphorothioate in the Sp configuration but were blocked by the Rp diastereomer. Both steps also proceeded with inversion of stereochemical configuration around phosphorus, consistent with a concerted transesterification reaction. These results are identical to those found for nuclear precursor mRNA (pre-mRNA) splicing and provide support for the hypothesis that group II introns and nuclear pre-mRNA introns share a common evolutionary history.

引用

页码：1685 / 1688

页数：4

共 23 条

[1] ABSOLUTE-CONFIGURATION OF DIASTEREOMERS OF ADENOSINE 5'-O-(1-THIOTRIPHOSPHATE) - CONSEQUENCES FOR STEREOCHEMISTRY OF POLYMERIZATION BY DNA-DEPENDENT RNA-POLYMERASE FROM ESCHERICHIA-COLI [J].