SPECTROFLUOROMETRIC METHOD FOR NO DETERMINATION

被引：58

作者：

GABOR, G

ALLON, N

机构：

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 220卷 / 01期

关键词：

D O I：

10.1006/abio.1994.1292

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A spectroscopic method for the quantitative determination of NO in aqueous solution is presented. The method is based on the uv absorbance of nitrosothiols, coupled with the inner filter effect. A threshold of 5.10(-7) M with a wide dynamic range of 5.10(-7)-10(-4) M are obtained. This method enables measurements in brain homogenates. Ultimately, since this method is immune to electrical perturbances, it will be applied at the tip of an optical fiber for in situ monitoring of NO or glutathione in brain and blood vessels. (C) 1994 Academic Press,Inc.

引用

页码：16 / 19

页数：4

共 23 条

[1]

CHOI KK, 1990, ANALYST, V105, P241

[2] HOMOGENEOUS CHEMILUMINESCENT MEASUREMENT OF NITRIC OXIDE WITH OZONE - IMPLICATIONS FOR CONTINUOUS SELECTIVE MONITORING OF GASEOUS AIR POLLUTANTS [J].