AN ARABINOSE-INDUCIBLE EXPRESSION VECTOR, PAR3, COMPATIBLE WITH COLE1-DERIVED PLASMIDS

被引：57

作者：

PEREZPEREZ, J ^{[1
]}

GUTIERREZ, J ^{[1
]}

机构：

[1] PHARMACIA ANTIBIOT FARMA SA,DEPT INVEST,E-28026 MADRID,SPAIN

来源：

GENE | 1995年 / 158卷 / 01期

关键词：

RECOMBINANT DNA; ESCHERICHIA COLI; PROTEIN SECRETION; CHAPERONIN;

D O I：

10.1016/0378-1119(95)00127-R

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Arabinose-inducible genetic elements from the Salmonella typhimurium arabinose operon were inserted into pACYC184. The resultant plasmid, pAR3, is compatible with ColE1-derived plasmids and allows efficient expression of recombinant (re) genes upon induction with arabinose. These features make it convenient for use in combination with standard gene expression vectors for the independently controlled production of two or more re-polypeptides in Escherichia coli.

引用

页码：141 / 142

页数：2

共 6 条

[1] PRODUCTION AND SECRETION OF HUMAN INTERLEUKIN-6 INTO THE PERIPLASM OF ESCHERICHIA-COLI - EFFICIENT PROCESSING OF N-TERMINAL VARIANTS OF HIL6 BY THE ESCHERICHIA-COLI SIGNAL PEPTIDASE [J].

BARTHELEMY, I ;

DEBUITRAGO, GG ;

CARREIRO, C ;

RONCAL, F ;

PEREZARANDA, A ;

MARQUEZ, G ;

BARBERO, JL .

JOURNAL OF BIOTECHNOLOGY, 1993, 27 (03) :307-316

[2] PRLA (SECY) AND PRLG (SECE) INTERACT DIRECTLY AND FUNCTION SEQUENTIALLY DURING PROTEIN TRANSLOCATION IN ESCHERICHIA-COLI [J].

BIEKER, KL ;

SILHAVY, TJ .

CELL, 1990, 61 (05) :833-842

[3] A NEW FAMILY OF SUGAR-INDUCIBLE EXPRESSION VECTORS FOR ESCHERICHIA-COLI [J].

CAGNON, C ;

VALVERDE, V ;

MASSON, JM .

PROTEIN ENGINEERING, 1991, 4 (07) :843-847

[4] DNA-SEQUENCE OF THE ARABAD-ARAC CONTROLLING REGION IN SALMONELLA-TYPHIMURIUM LT2 [J].

HORWITZ, AH ;

HEFFERNAN, L ;

MORANDI, C ;

LEE, JH ;

TIMKO, J ;

WILCOX, G .

GENE, 1981, 14 (04) :309-319

[5] NOVEL HIGH-LEVEL EXPRESSION CLONING VEHICLES - 10(4)-FOLD AMPLIFICATION OF ESCHERICHIA-COLI MINOR PROTEIN [J].

MASUI, Y ;

MIZUNO, T ;

NOUYE, M .

BIO-TECHNOLOGY, 1984, 2 (01) :81-85

[6] THE UNIVERSALLY CONSERVED GROE (HSP60) CHAPERONINS [J].

ZEILSTRARYALLS, J ;

FAYET, O ;

GEORGOPOULOS, C .

ANNUAL REVIEW OF MICROBIOLOGY, 1991, 45 :301-325

← 1 →