SUPERCRITICAL FLUID CHROMATOGRAPHY LIGHT-SCATTERING DETECTOR - A PROMISING COUPLING FOR POLAR COMPOUNDS ANALYSIS WITH PACKED-COLUMNS

被引：55

作者：

CARRAUD, P ^{[1
]}

THIEBAUT, D ^{[1
]}

CAUDE, M ^{[1
]}

ROSSET, R ^{[1
]}

LAFOSSE, M ^{[1
]}

DREUX, M ^{[1
]}

机构：

[1] UNIV ORLEANS,CHIM ORGAN PHYS & CHROMATOG LAB,F-45067 ORLEANS 2,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1987年 / 25卷 / 09期

关键词：

LIGHT; -; Scattering; METHANOL;

D O I：

10.1093/chromsci/25.9.395

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The coupling of carbon dioxide supercritical fluid chromatography (SFC) on packed columns with a lightscattering detector (LSD) is investigated. The fused-silica capillary restrictor acts as nebulizer. The temperature of the restrictor and the vaporization tube allows the use of polar modifiers such as methanol in the supercritical CO//2. Detection limit is five times higher than with HPLC. With packed columns, LSD is complementary to the flame ionization detector (FID). Several examples of applications are given.

引用

页码：395 / 398

页数：4

共 15 条

[1]

ASMUS PA, 1984, J CHROMATOGR, V316, P461

[2] EVAPORATIVE ANALYZER AS A MASS DETECTOR FOR LIQUID-CHROMATOGRAPHY [J].

CHARLESWORTH, JM .

ANALYTICAL CHEMISTRY, 1978, 50 (11) :1414-1420

[3]

LAFOSSE M, 1986, 16TH INT S CHROM PAR

[4]

LAFOSSE M, 1986, 10TH INT S COL LIQ C

[5] INVESTIGATIONS OF THE USES OF MODIFIERS IN SUPERCRITICAL FLUID CHROMATOGRAPHY [J].

LEVY, JM ;

RITCHEY, WM .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1986, 24 (06) :242-248

[6] PRINCIPLES OF OPERATION OF AN EVAPORATIVE LIGHT-SCATTERING DETECTOR FOR LIQUID-CHROMATOGRAPHY [J].

MOUREY, TH ;

OPPENHEIMER, LE .

ANALYTICAL CHEMISTRY, 1984, 56 (13) :2427-2434

[7] SUPERCRITICAL AND SUBCRITICAL FLUID CHROMATOGRAPHY ON A CHIRAL STATIONARY PHASE FOR THE RESOLUTION OF PHOSPHINE OXIDE ENANTIOMERS [J].

MOURIER, PA ;

ELIOT, E ;

CAUDE, MH ;

ROSSET, RH ;

TAMBUTE, AG .

ANALYTICAL CHEMISTRY, 1985, 57 (14) :2819-2823

[8]

NOVOTNY M, 1986, HRC-J HIGH RES CHROM, V9, P137

[9] EXAMINATION OF THE CONCENTRATION RESPONSE OF EVAPORATIVE LIGHT-SCATTERING MASS DETECTORS [J].

OPPENHEIMER, LE ;

MOUREY, TH .

JOURNAL OF CHROMATOGRAPHY, 1985, 323 (02) :297-304

[10]

PERRIN JL, 1984, REV FR CORPS GRAS, V31, P495

← 1 2 →