A NEW TECHNIQUE FOR EXPLANTATION AND INVITRO CULTIVATION OF CHICKEN EMBRYOS

被引：56

作者：

DUGAN, JD

LAWTON, MT

GLASER, B

BREM, H

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROL SURG,BALTIMORE,MD 21205

[2] JOHNS HOPKINS UNIV,SCH MED,DEPT OPHTHALMOL,BALTIMORE,MD 21205

[3] JOHNS HOPKINS UNIV,SCH MED,DEPT ONCOL,BALTIMORE,MD 21205

[4] ST JOSEPHS HOSP,CTR RETINA,TOWSON,MD

来源：

ANATOMICAL RECORD | 1991年 / 229卷 / 01期

关键词：

D O I：

10.1002/ar.1092290114

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

A technique is described for explanting and cultivating chicken embryos in plastic drinking cups which have been modified with plastic wrap to reproduce the geometry and dimensions of the egg shell. Successful explantation rates of 97% are possible with a double‐window technique, and survivability in cups exceeds that achievable in other in vitro systems (i.e., petri dishes). Longterm survival to the 21st day of incubation is seen routinely. This system with cups is less expensive than that with petri dishes, and simpler than that with plastic wrap/tripods. Thus, this new method of in vitro cultivation of chicken embryos improves upon explantation rate, survivability and system design, and has a wide range of applications in developmental biology, angiogenesis, cancer, and pharmacology research. Copyright © 1991 Wiley‐Liss, Inc.

引用

页码：125 / 128

页数：4

共 13 条

[1] SIMPLE PROCEDURE FOR LONG-TERM CULTIVATION OF CHICKEN EMBRYOS [J].