POLY(METHYLGLUTAMATE)-COATED SURFACES IN HPLC AND CE

被引：45

作者：

BENTROP, D ^{[1
]}

KOHR, J ^{[1
]}

ENGELHARDT, H ^{[1
]}

机构：

[1] UNIV SAARLAND,W-6600 SAARBRUCKEN,GERMANY

来源：

CHROMATOGRAPHIA | 1991年 / 32卷 / 3-4期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; CAPILLARY ELECTROPHORESIS; SURFACE COATING; POLY(METHYLGLUTAMATE);

D O I：

10.1007/BF02325022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Thermal polymerization of the N-carboxyanhydride of glutamic acid-5-methylester was used to coat microparticulate silica and the surface of capillaries with poly(methylglutamate)(PMG). By increasing the thickness of the PMG layer the hydrophobicity of the stationary phases could bc increased. However, total shielding of surface silanols was not achieved. The PMG-coated silicas for HPLC showed unexpected and strange behavior in the chromatography of proteins. Nevertheless, capillaries coated with PMG proved to be excellent for highly efficient CE separations of proteins at medium pH values.

引用

页码：171 / 178

页数：8

共 56 条

[1] CATION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PROTEINS ON POLY(ASPARTIC ACID) SILICA [J].