NYSTATIN CHANGES THE PROPERTIES OF TRANSPORTERS FOR ARGININE AND SUGARS - AN IN-VITRO STUDY

被引：18

作者：

OPEKAROVA, M ^{[1
]}

TANNER, W ^{[1
]}

机构：

[1] UNIV REGENSBURG, LEHRSTUHL ZELLBIOL & PFLANZENPHYSIOL, D-93040 REGENSBURG, GERMANY

来源：

FEBS LETTERS | 1994年 / 350卷 / 01期

关键词：

NYSTATIN; YEAST PLASMA MEMBRANE VESICLES; ARGININE TRANSPORT;

D O I：

10.1016/0014-5793(94)00730-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In ergosterol-containing energized yeast plasma membrane vesicles nystatin (5-10 mu g/mg total lipid) caused a massive efflux of pre-accumulated arginine while the membrane potential (the principal driving force; -110 mV) decreased by only 10-30 mV. Neither the substrate fluxes nor the membrane potential was influenced by nystatin when the permease was reconstituted in ergosterol-free phospholipid vesicles. The same effect of nystatin was found with the reconstituted sugar transporter from Chlorella kessleri. It is suggested that nystatin binding to ergosterol in the vicinity of the permease releases the transport protein from its coupling to energy and converts it to a facilitator.

引用

页码：46 / 50

页数：5

共 17 条

[1] HOW DO THE POLYENE MACROLIDE ANTIBIOTICS AFFECT THE CELLULAR MEMBRANE-PROPERTIES [J].

BOLARD, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 864 (3-4) :257-304

[2] POTASSIUMLESS DEATH OF SACCHAROMYCES CEREVISIAE CELLS TREATED WITH N-SUCCINYL PERIMYCIN AND REVERSAL OF FUNGICIDAL ACTION OF ANTIBIOTIC BY POTASSIUM IONS [J].

BOROWSKI, E ;

CYBULSKA, B .

NATURE, 1967, 213 (5080) :1034-&

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] INFLUENCE OF EXTRACELLULAR K+ OR MG-2+ ON THE STAGES OF THE ANTIFUNGAL EFFECTS OF AMPHOTERICIN-B AND FILIPIN [J].

BRAJTBURG, J ;

MEDOFF, G ;

KOBAYASHI, GS ;

ELBERG, S .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1980, 18 (04) :593-597

[5]

CASPARI T, 1994, J BIOL CHEM, V269, P3498

[6] DISSOCIATION BETWEEN ION PERMEABILITY AND LETHAL ACTION OF POLYENE ANTIBIOTICS ON CANDIDA-ALBICANS [J].

CHEN, WC ;

CHOU, DL ;

FEINGOLD, DS .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1978, 13 (06) :914-917

[7]

GOFFEAU A, 1988, METHOD ENZYMOL, V157, P528

[8] AMINO-ACID-TRANSPORT IN EUKARYOTIC MICROORGANISMS [J].

HORAK, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 864 (3-4) :223-256

[9]

HSUCHEN CC, 1974, NATURE, V251, P658

[10] ANTIFUNGAL AGENTS - RECENT DEVELOPMENTS [J].

KOBAYASHI, GS ;

MEDOFF, G .

ANNUAL REVIEW OF MICROBIOLOGY, 1977, 31 :291-308

← 1 2 →