HIGH-RESOLUTION VISUALIZATION BY FIELD-EMISSION SCANNING ELECTRON-MICROSCOPY OF ENTEROCOCCUS-FAECALIS SURFACE-PROTEINS ENCODED BY THE PHEROMONE-INDUCIBLE CONJUGATIVE PLASMID PCF10

被引:44
作者
OLMSTED, SB
ERLANDSEN, SL
DUNNY, GM
WELLS, CL
机构
[1] UNIV MINNESOTA, DEPT LAB MED & PATHOL, MINNEAPOLIS, MN 55455 USA
[2] UNIV MINNESOTA, DEPT CELL BIOL & NEUROANAT, MINNEAPOLIS, MN 55455 USA
[3] UNIV MINNESOTA, DEPT MICROBIOL, MINNEAPOLIS, MN 55455 USA
[4] UNIV MINNESOTA, INST ADV STUDIES BIOL PROC TECHNOL, MINNEAPOLIS, MN 55455 USA
关键词
D O I
10.1128/JB.175.19.6229-6237.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Enterococcus faecalis can acquire antibiotic resistance and virulence genes by transfer of pheromone-inducible conjugative plasmids such as pCF10, which encodes tetracycline resistance. Two pCF10-encoded cell surface proteins, Sec10 and Asc10, have been previously shown to play an important role in the transfer of this plasmid. We used high-resolution, field emission scanning electron microscopy to visualize these proteins on the surfaces of a series of isogenic strains of E. faecalis. Immunogold labeling, using both 6- and 12-nm colloidal gold, unambiguously demonstrated the expression and distribution of Sec10 and Asc10 on the surface of the E. faecalis cells. On unlabeled E. faecalis cells which expressed either Sec10 or Asc10, the former appeared to be more readily detected. Immunogold labeling of E. faecalis cells expressing both Asc10 and Sec10 clearly demonstrated the abundance and intermixing of both proteins on the cell surface except at septal regions. Sec10 was observed to be distributed over the cell surface. At regions of cell-cell contact, fine strands representing Asc10 were observed directly attaching adjacent cells to one another.
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收藏
页码:6229 / 6237
页数:9
相关论文
共 37 条
[1]   ANTIGENIC COMPOSITION OF AN ENDOCARDITIS-ASSOCIATED ISOLATE OF STREPTOCOCCUS-FAECALIS AND IDENTIFICATION OF ITS GLYCOPROTEIN ANTIGENS BY LIGAND BLOTTING WITH LECTINS [J].
AITCHISON, EJ ;
LAMBERT, PA ;
FARRELL, ID .
JOURNAL OF MEDICAL MICROBIOLOGY, 1986, 21 (02) :161-167
[2]   SERODIAGNOSIS OF STREPTOCOCCUS-FAECALIS ENDOCARDITIS BY IMMUNOBLOTTING OF SURFACE PROTEIN ANTIGENS [J].
AITCHISON, EJ ;
LAMBERT, PA ;
SMITH, EG ;
FARRELL, ID .
JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (02) :211-215
[3]   TECHNIQUES FOR THE PRESERVATION OF 3-DIMENSIONAL STRUCTURE IN PREPARING SPECIMENS FOR THE ELECTRON MICROSCOPE [J].
ANDERSON, TF .
TRANSACTIONS OF THE NEW YORK ACADEMY OF SCIENCES, 1951, 13 (04) :130-134
[4]  
BOYDE A, 1981, SCAN ELECTRON MICROS, P27
[5]  
BOYDE A, 1979, Scanning, V2, P149
[6]   CLONING AND EXPRESSION OF GENES ENCODING PHEROMONE-INDUCIBLE ANTIGENS OF ENTEROCOCCUS-(STREPTOCOCCUS)-FAECALIS [J].
CHRISTIE, PJ ;
KAO, SM ;
ADSIT, JC ;
DUNNY, GM .
JOURNAL OF BACTERIOLOGY, 1988, 170 (11) :5161-5168
[7]   SEX-PHEROMONES AND PLASMID TRANSFER IN ENTEROCOCCUS-FAECALIS [J].
CLEWELL, DB ;
WEAVER, KE .
PLASMID, 1989, 21 (03) :175-184
[8]   CELL WALL REPLICATION IN STREPTOCOCCUS PYOGENES - IMMUNOFLUORESCENT METHODS APPLIED DURING GROWTH SHOW THAT NEW WALL IS FORMED EQUATORIALLY [J].
COLE, RM ;
HAHN, JJ .
SCIENCE, 1962, 135 (3505) :722-&
[9]   DIRECT STIMULATION OF THE TRANSFER OF ANTIBIOTIC-RESISTANCE BY SEX-PHEROMONES IN STREPTOCOCCUS-FAECALIS [J].
DUNNY, G ;
FUNK, C ;
ADSIT, J .
PLASMID, 1981, 6 (03) :270-278
[10]   GENETIC FUNCTIONS AND CELL CELL-INTERACTIONS IN THE PHEROMONE-INDUCIBLE PLASMID TRANSFER SYSTEM OF ENTEROCOCCUS-FAECALIS [J].
DUNNY, GM .
MOLECULAR MICROBIOLOGY, 1990, 4 (05) :689-696