SEPARATION AND DETECTION OF ACIDIC AND NEUTRAL IMPURITIES IN ILLICIT HEROIN VIA CAPILLARY ELECTROPHORESIS

被引：57

作者：

LURIE, IS ^{[1
]}

CHAN, KC ^{[1
]}

SPRATLEY, TK ^{[1
]}

CASALE, JF ^{[1
]}

ISSAQ, HJ ^{[1
]}

机构：

[1] NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC DYNCORP,FREDERICK,MD 21702

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 669卷 / 01期

关键词：

D O I：

10.1016/0378-4347(95)00148-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation and detection of acidic and neutral impurities in illicit heroin using capillary electrophoresis (CE) is described. Separations were achieved using charged cyclodextrin modified micellar electrokinetic capillary chromatography. The use of the anionic beta-cyclodextrin sulfobutyl ether 1V in combination with sodium dodecyl sulfate significantly increased resolution. Improved selectivity and/or sensitivity in detection was obtained using photodiode array ultraviolet and laser-induced fluorescence detection. The phenanthrene-like heroin impurities exhibit high native fluorescence under krypton-fluoride laser excitation (248 nm). The limit of detection by laser-induced fluorescence detection for one of these solutes (acetylthebaol) is 1.8 ng/ml, 500 times more sensitive than UV. This methodology is applicable to analysis of both crude and refined heroin.

引用

页码：3 / 13

页数：11

共 16 条

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