THE BIOSYNTHESIS OF LEWIS-X IN HELICOBACTER-PYLORI

被引：62

作者：

CHAN, NWC ^{[1
]}

STANGIER, K ^{[1
]}

SHERBURNE, R ^{[1
]}

TAYLOR, DE ^{[1
]}

ZHANG, YN ^{[1
]}

DOVICHI, NJ ^{[1
]}

PALCIC, MM ^{[1
]}

机构：

[1] UNIV ALBERTA, DEPT MED MICROBIOL & INFECT DIS, EDMONTON, AB T6G 2H7, CANADA

来源：

GLYCOBIOLOGY | 1995年 / 5卷 / 07期

基金：

加拿大自然科学与工程研究理事会;

关键词：

FUCOSYL-TRANSFERASE; GALACTOSYLTRANSFERASE; HELICOBACTER PYLORI; LEWIS X;

D O I：

10.1093/glycob/5.7.683

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biosynthesis of the Lewis X determinant (Gal beta 1-4 [Fuc alpha 1-3]GlcNAc beta-) in three strains of Helicobacter pylori has been investigated, Strains UA 861, UA 802 and UA 1182 contain alpha 1,3 fucosyltransferase and beta 1,4 galactosyltransferase activities that synthesize the Lewis X structure by the transfer of monosaccharides from GDP-fucose and UDP-gaIactose donors, respectively. The enzyme reaction products that formed were characterized by capillary zone electrophoresis and by H-1-NMR spectroscopy. The biosynthetic pathway is therefore identical to that found in humans, In the three strains, the fucosyltransferase and galactosyltransferase activities differed in various cellular fractions, K-m values for their donor and acceptor substrates also differed.

引用

页码：683 / 688

页数：6

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