REAL-TIME IMAGING OF INTRACELLULAR CALCIUM CHANGE WITH SIMULTANEOUS SINGLE CHANNEL RECORDING IN MAMMARY EPITHELIAL-CELLS

被引：21

作者：

FURUYA, K ^{[1
]}

ENOMOTO, K ^{[1
]}

机构：

[1] SHIMANE MED UNIV,DEPT PHYSIOL,IZUMO,SHIMANE 693,JAPAN

来源：

BRAIN RESEARCH BULLETIN | 1990年 / 25卷 / 05期

关键词：

Calcium oscillation; Calcium-activated K[!sup]+[!/sup] channel; Fura-2; Image processing; Intracellular calcium; Mammary epithelial cells; Mechanical stimulation; Patch clamp;

D O I：

10.1016/0361-9230(90)90058-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A method of continuous image subtraction of fura-2 fluorescence made it possible to observe real-time (video rate) changes in intracellular calcium (Cai). This simple method is very useful for simultaneous measurements in electrophysiology with a system containing cells of different states. A method for synchronous recording of Cai change and single channel activity is also described. In cultured mammary epithelial cells, these methods revealed a propagating Cai signal induced by mechanical stimulation and spontaneous Cai oscillation with synchronous activation of calcium-activated potassium channels. © 1990.

引用

页码：779 / 781

页数：3

共 5 条

[1] ENOMOTO K, 1987, FEBS LETT, V203, P82
[2] INDUCTION OF DISTINCT TYPES OF SPONTANEOUS ELECTRICAL-ACTIVITIES IN MAMMARY EPITHELIAL-CELLS BY EPIDERMAL GROWTH-FACTOR AND INSULIN
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[3] SINGLE CALCIUM-ACTIVATED POTASSIUM CHANNEL IN CULTURED MAMMARY EPITHELIAL-CELLS
FURUYA, K
ENOMOTO, K
FURUYA, S
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EDWARDS, C
OKA, T
[J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1989, 414 (02): : 118 - 124
[4] MEASUREMENT OF CYTOSOLIC FREE CA-2+ IN INDIVIDUAL SMALL CELLS USING FLUORESCENCE MICROSCOPY WITH DUAL EXCITATION WAVELENGTHS
TSIEN, RY
RINK, TJ
POENIE, M
[J]. CELL CALCIUM, 1985, 6 (1-2) : 145 - 157
[5] CALCIUM GRADIENTS IN SINGLE SMOOTH-MUSCLE CELLS REVEALED BY THE DIGITAL IMAGING MICROSCOPE USING FURA-2
WILLIAMS, DA
FOGARTY, KE
TSIEN, RY
FAY, FS
[J]. NATURE, 1985, 318 (6046) : 558 - 561

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