EFFECT OF OXYGEN-SUPPLY ON THE SUSPENSION-CULTURE OF GENETICALLY MODIFIED TOBACCO CELLS

被引：36

作者：

GAO, JW ^{[1
]}

LEE, JM ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV,DEPT CHEM ENGN,PULLMAN,WA 99164

来源：

BIOTECHNOLOGY PROGRESS | 1992年 / 8卷 / 04期

关键词：

D O I：

10.1021/bp00016a004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of oxygen supply on the cultivation of the genetically modified tobacco cells and the formation of a foreign protein, beta-glucuronidase (GUS), was investigated in 250-mL Erleneyer flasks, a 5-L stirred tank fermenter, and a 7-L air-lift fermenter. The oxygen supply was varied by using different volumes of medium in the case of the 250-mL Erlenmeyer flask culture or by the different aeration rate in the case of the two types of fermenters tested. Higher oxygen supply stimulated cell growth and increased oxygen consumption rate, the level of phenolics, and GUS productions.

引用

页码：285 / 290

页数：6

共 27 条

[1] DEVELOPMENT OF PLANT PROMOTER EXPRESSION VECTORS AND THEIR USE FOR ANALYSIS OF DIFFERENTIAL ACTIVITY OF NOPALINE SYNTHASE PROMOTER IN TRANSFORMED TOBACCO CELLS [J].