COMPARISON OF THE 2ND INTERNAL TRANSCRIBED SPACER (RIBOSOMAL DNA) FROM POPULATIONS AND SPECIES OF FASCIOLIDAE (DIGENEA)

被引：155

作者：

ADLARD, RD

BARKER, SC

BLAIR, D

CRIBB, TH

机构：

[1] QUEENSLAND MUSEUM,BRISBANE,QLD 4101,AUSTRALIA

[2] JAMES COOK UNIV N QUEENSLAND,DEPT ZOOL,TOWNSVILLE,QLD 4811,AUSTRALIA

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1993年 / 23卷 / 03期

基金：

澳大利亚研究理事会;

关键词：

FASCIOLA-HEPATICA; FASCIOLA-GIGANTICA; FASCIOLOIDES-MAGNA; RDNA; RIBOSOMAL DNA; INTERNAL TRANSCRIBED SPACER; ITS; PCR;

D O I：

10.1016/0020-7519(93)90022-Q

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The nucleotide sequence of the 3' end of the second internal transcribed spacer region (ITS2) was determined for four isolates of Fasciola hepatica, two isolates of Fasciola gigantica, one isolate of Fascioloides magna and one isolate of Fasciola sp. from Japan. Intraspecific sequence divergence was negligible. Sequence divergence between F. hepatica and F. gigantica was 2.8% and between Fasciola hepatica and Fascioloides magna was 13.2%. The sequence of Fasciola sp. from Japan matched closely that of F. gigantica. This study demonstrates variability in nucleotide sequence within the ITS2 region which allows discrimination between species of Fasciolidae.

引用

页码：423 / 425

页数：3

共 9 条

[1] RESTRICTION ENZYME MAPPING OF RIBOSOMAL DNA CAN DISTINGUISH BETWEEN FASCIOLID (LIVER FLUKE) SPECIES [J].