The effects of the Kampo medicine Toki-Shakuyaku-San on the motility and learning ability of mice were studied. An assay on mice aged 5 months after 15 weeks of administration revealed no change in spontaneous motility. However, in a study on learning ability employing passive avoidance task, the latent periods of test trial were significantly longer in the Toki-Shakuyaku-San administered group than in a control group, indicating that Toki-Shakuyaku-San may play some role in the alleviation of learning disturbances in mice.