O2-POLAROGRAPHIC STUDIES ON SOLUBLE AND MITOCHONDRIAL ENZYMES OF CRITHIDIA FASCICULATA - GLYCEROPHOSPHATE ENZYMES

被引：22

作者：

BACCHI, CJ

HUTNER, SH

CIACCIO, EI

MARCUS, SM

机构：

[1] Haskins Laboratories, New York, New York, 10017

[2] Dept of Biological Sciences, Fordham Univ, Bronx, New York

[3] Dept of Pharmacology, Hahnemann Medical College, Philadelphia, Pennsylvania

来源：

JOURNAL OF PROTOZOOLOGY | 1968年 / 15卷 / 03期

关键词：

D O I：

10.1111/j.1550-7408.1968.tb02175.x

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

SYNOPSIS. Mitochondrial and supernatant fractions were isolated from Crithidia fasciculata by grinding with neutral alumina and differential centrifugation. Supernatant fractions contained at least 2 NAD‐linked enzymes: an α‐glycerophosphate dehydrogenase and a malate dehydrogenase. The properties of these enzymes were investigated polarographically with phenazine ethosulfate acting as electron acceptor. Agaricic acid, cinnamic acid and p‐NO2‐cinnamic acid were specific inhibitors of the α‐glycerophosphate dehydrogenase. Succinate, malate, DL‐α‐glycerophosphate and NADH stimulated respiration of mitochondrial preparations; O2 uptake was greatest with succinate. KCN and antimycin A inhibited succinate respiration more than α‐glycerophosphate respiration. Amytal did not affect succinate, α‐glycerophosphate or NADH oxidation. The trypanocide suramin inhibited mitochondrial respiration at least 77% with each substrate. The relevance of these results to other members of the Trypanosomatidae is discussed. Copyright © 1968, Wiley Blackwell. All rights reserved

引用

页码：576 / &

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