ROLE OF MULTIPLE BINDING SITES IN INHIBITION OF NADH OXIDASE BY PIERICIDIN AND ROTENONE

被引:12
作者
GUTMAN, M
SINGER, TP
CASIDA, JE
机构
[1] Molecular Biology Division, Veterans Administration Hospital, San Francisco
[2] Department of Biochemistry and Biophysics, University of California Medical Center, San Francisco
[3] Division of Entomology, University of California, Berkeley
基金
美国国家科学基金会;
关键词
D O I
10.1016/0006-291X(69)90854-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Scatchard plots indicate that 14C-piericidin A and -rotenone bind at 2 specific sites per mole of NADH dehydrogenase in ETP, but the titer found for complex I or mersalyl-treated ETP more closely approximates 1. The curves for inhibition of NADH oxidase by piericidin and rotenone are sigmoidal; this results from an unequal contribution of the 2 specific sites to the inhibition. An unspecific binding site also contributes to the inhibition in a manner reversed by washing the particles with bovine serum albumin (BSA). In contrast, inhibition of NADH-CoQ reductase activity is due entirely to binding at specific site(s) because BSA does not restore activity. © 1969.
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页码:615 / &
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