HETERODIMERIC CAPPING PROTEINS CONSTITUTE A HIGHLY CONSERVED GROUP OF ACTIN-BINDING PROTEINS

被引：19

作者：

HARTMANN, H ^{[1
]}

SCHLEICHER, M ^{[1
]}

NOEGEL, AA ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY

来源：

DEVELOPMENTAL GENETICS | 1990年 / 11卷 / 5-6期

关键词：

CYTOSKELETON; CAPPING PROTEINS; GENOMIC STRUCTURE; DICTYOSTELIUM;

D O I：

10.1002/dvg.1020110508

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The two subunits of the heterodimeric protein cap32/34, an actin-binding protein, are encoded by separate single-copy genes. We have established the genomic structure of both genes. A sequence comparison of cap32/34 with capZ from chicken skeletal muscle and two partially known sequences from Saccharomyces cerevisiae and Xenopus laevis show that heterodimeric capping proteins belong to a highly conserved group of actin-binding proteins. This conclusion is supported by the cross-reacting of polyclonal antibodies against cap32 and cap34 with proteins from lower and higher eukaryotes. In addition, a system is presented that allows the expression of truncated cap34 polypeptides under the control of the cap34 promoter.

引用

页码：369 / 376

页数：8

共 42 条

[1] A DICTYOSTELIUM MUTANT DEFICIENT IN SEVERIN, AN F-ACTIN FRAGMENTING PROTEIN, SHOWS NORMAL MOTILITY AND CHEMOTAXIS [J].