SGS-3 CHROMATIN STRUCTURE AND TRANS-ACTIVATORS - DEVELOPMENTAL AND ECDYSONE INDUCTION OF A GLUE ENHANCER-BINDING FACTOR, GEBF-I, IN DROSOPHILA LARVAE

被引：32

作者：

GEORGEL, P ^{[1
]}

RAMAIN, P ^{[1
]}

GIANGRANDE, A ^{[1
]}

DRETZEN, G ^{[1
]}

RICHARDS, G ^{[1
]}

BELLARD, M ^{[1
]}

机构：

[1] FAC MED STRASBOURG, INST CHIM BIOL,CNRS,GENET MOLEC EUCARYOTES LAB, INSERM,U184, F-67085 STRASBOURG, FRANCE

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1991年 / 11卷 / 01期

关键词：

D O I：

10.1128/MCB.11.1.523

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transcription of the Drosophila melanogaster 68C salivary gland glue gene Sgs-3 involves the interaction of a distal and a proximal regulatory region. These are marked in vivo by a specific chromatin structure which is established sequentially during development, starting early in embryogenesis. The distal region is characterized by a stage- and tissue-specific DNase I hypersensitive site. A stage- and tissue-specific factor, GEBF-I, binds in this region and is missing in 2B5 mutant larvae which lack Sgs-3 transcripts. This binding involves the simultaneous interaction with two distinct DNA sequences which induces conformational changes in the protein. Salivary glands acquire competence to respond to ecdysone in the mid-third larval instar, whereafter the hormone rapidly induces both the GEBF-I protein and Sgs-3 transcription.

引用

页码：523 / 532

页数：10

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