CHARACTERIZATION OF FULL-LENGTH CDNAS AND THE GENE CODING FOR THE HUMAN GM2 ACTIVATOR PROTEIN

被引：46

作者：

KLIMA, H

TANAKA, A

SCHNABEL, D

NAKANO, T

SCHRODER, M

SUZUKI, K

SANDHOFF, K

机构：

[1] INST ORGAN CHEM & BIOCHEM,GERHARD DOMAGK STR 1,W-5300 BONN 1,GERMANY

[2] UNIV N CAROLINA,SCH MED,BRAIN & DEV RES CTR,CHAPEL HILL,NC 27599

来源：

FEBS LETTERS | 1991年 / 289卷 / 02期

关键词：

GM2-ACTIVATOR PROTEIN; GENOMIC STRUCTURE; GLYCOLIPID-BINDING PROTEIN;

D O I：

10.1016/0014-5793(91)81084-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Full-length cDNAs coding for the human GM2-activator protein has been isolated and characterized, and its genomic structure studied in two overlapping clones in lambda-EMBL-4 isolated from a human brain genomic library. Two different cDNAs were found that were identical to the 5'-terminus to nt 1311 (counted from the A of the initiation codon, ATG) including the entire protein coding sequence. However, they were entirely dissimilar in the 3'-non-coding sequences. The genomic clones covered 94% of the full-length cDNA sequence. Three introns were found. The last exon spans contiguously the carboxyl terminus of the protein and the entire 3'-untranslated region of one of the two cDNAs with different 3'-ends. The origin of the 3'-portion of the other cDNA clone is not clear at this time.

引用

页码：260 / 264

页数：5

共 11 条

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