EXPRESSION OF MUSCARINIC AND NICOTINIC RECEPTOR MESSENGER-RNA IN THE SALIVARY-GLAND OF RATS - A STUDY BY INSITU HYBRIDIZATION HISTOCHEMISTRY

被引：9

作者：

SHIDA, T

TOKUNAGA, A

KONDO, E

UEDA, Y

OHNO, K

SAIKA, T

KIYAMA, H

TOHYAMA, M

机构：

[1] OSAKA UNIV,SCH MED,DEPT OTOLARYNGOL,OSAKA,JAPAN

[2] OSAKA UNIV,SCH MED,DEPT ANAT & NEUROSCI 2,OSAKA,JAPAN

来源：

MOLECULAR BRAIN RESEARCH | 1993年 / 17卷 / 3-4期

基金：

日本科学技术振兴机构;

关键词：

ACETYLCHOLINE; MUSCARINIC RECEPTOR; NICOTINIC RECEPTOR; MESSENGER RNA; SALIVARY GLAND; INSITU HYBRIDIZATION; RAT;

D O I：

10.1016/0169-328X(93)90019-L

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Expression of muscarinic receptor mRNA subtypes (m1-5) and nicotinic receptor subunits (alpha2-4, and beta2) was examined in the rat submandibular gland by in situ hybridization histochemistry, using oligonucleotide probes for the muscarinic receptor and RNA probes for the nicotinic receptors. m2, alpha3, and beta2 mRNA were strongly expressed in the submandibular ganglion, and m3, alpha2, alpha3, alpha4, and beta2 were expressed in the striated and interlobular duct cells. Both muscarinic and nicotinic receptors were coexpressed in the same ganglion neurons, while none of these mRNA were detected in the terminal secretory units.

引用

页码：335 / 339

页数：5

共 17 条

[1]

BHASKAR SN, 1989, ORAL HISTOLOGY EMBRI, P337

[2] INSITU HYBRIDIZATION HISTOCHEMISTRY FOR THE ANALYSIS OF GENE-EXPRESSION IN THE ENDOCRINE AND CENTRAL-NERVOUS-SYSTEM TISSUES - A 3-YEAR EXPERIENCE [J].