THE RAT BETA-3-ADRENERGIC RECEPTOR GENE CONTAINS AN INTRON

被引：41

作者：

BENSAID, M ^{[1
]}

KAGHAD, M ^{[1
]}

RODRIGUEZ, M ^{[1
]}

LEFUR, G ^{[1
]}

CAPUT, D ^{[1
]}

机构：

[1] SANOFI ELF BIORECH,BIOL MOLEC LAB,BP 137,F-31676 LABEGE,FRANCE

来源：

FEBS LETTERS | 1993年 / 318卷 / 03期

关键词：

BETA-3-ADRENERGIC RECEPTOR; INTRON; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0014-5793(93)80516-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report that the rat beta3-adrenergic receptor (beta3-AR) gene has an intron. The intron starts with an in-frame stop codon with the result that unspliced transcripts will encode a C-terminal truncated protein. The reported protein sequences of mouse and human beta3-AR were both deduced from genomic DNA sequences. Given the heterogeneity at the C-termini of the otherwise highly similar rat, mouse and human sequences, we discuss the intriguing possibility that the beta3-AR gene of the latter two species also contain an intron near the extremity of the open reading frame. A beta-adrenergic receptor (beta-AR) cDNA we have cloned from rat colonic tissue which has a sequence essentially identical to that previously reported for the rat adipose beta3-AR cDNA [(1991) J. Chem. 266, 24053], encodes the spliced version of the beta3-AR.

引用

页码：223 / 226

页数：4

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