AMINO-ACID-SEQUENCE OF A TRYPSIN/CHYMOTRYPSIN INHIBITOR FROM GIANT TARO (ALOCASIA-MACRORRHIZA)

被引:12
作者
ARGALL, ME
BRADBURY, JH
SHAW, DC
机构
[1] AUSTRALIAN NATL UNIV,DEPT CHEM,CANBERRA,ACT 0200,AUSTRALIA
[2] AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,PROT BIOCHEM GRP,CANBERRA,ACT 2601,AUSTRALIA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1994年 / 1204卷 / 02期
关键词
PROTEIN SEQUENCE; TRYPSIN/CHYMOTRYPSIN INHIBITOR; POSITIONAL IDENTITY; KUNITZ-TYPE INHIBITOR; P-1; SITE; (GIANT TARO (A-MACRORRHIZA));
D O I
10.1016/0167-4838(94)90008-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Giant tare (Alocasia macrorrhiza) contains a protein which inhibits both trypsin and chymotrypsin. This trypsin/chymotrypsin inhibitor exists as a dimer of two identical monomers each with slight polymorphism and is an attractive candidate for conferring insect resistance in transgenic plants. The 184 amino-acid sequence (molecular mass of 19774 Da for the Met-24, Glu-50 form) has been determined and is compared with those of other Kunitz-type trypsin, chymotrypsin and subtilisin inhibitors. There appears to be greater 'homology' between the giant tare inhibitor and those inhibitors from other monocotyledons than inhibitors from dicotyledons. The P-1 loop region is different from that of other Kunitz-type inhibitors and contains a sequence Leu-Ala-Phe-Phe-Pro at residues 56-60. This section of sequence differs only by a Leu/Ile replacement to a tight binding inhibitor of neutrophil elastase, recently produced by genetic engineering. The most likely candidate for the P-1 residue in the giant tare trypsin/chymotrypsin inhibitor is Leu-56.
引用
收藏
页码:189 / 194
页数:6
相关论文
共 31 条
[1]  
ARGALL ME, 1987, THESIS AUSTR NATIONA
[2]   BOVINE BETA-LACTOGLOBULIN-E, BETA-LACTOGLOBULIN-F AND BETA-LACTOGLOBULIN-G OF BALI (BANTENG) CATTLE, BOS-(BIBOS)-JAVA']JAVANICUS [J].
BELL, K ;
MCKENZIE, HA ;
SHAW, DC .
AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES, 1981, 34 (02) :133-147
[3]  
Bradbury J.H., 1988, CHEM TROPICAL ROOT C, P201
[4]   COMPARATIVE-STUDY OF PROTEINASE-INHIBITORS IN TROPICAL ROOT CROPS AND SURVEY OF ALLELOCHEMICALS IN THE EDIBLE AROIDS [J].
BRADBURY, JH ;
HAMMER, BC .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1990, 38 (07) :1448-1453
[5]   ALPHA-1-ANTITRYPSIN AND THE SERPINS - VARIATION AND COUNTERVARIATION [J].
CARRELL, R ;
TRAVIS, J .
TRENDS IN BIOCHEMICAL SCIENCES, 1985, 10 (01) :20-24
[6]  
CHAN HT, 1983, HDB TROPICAL FOODS P
[7]  
CHANDRA S, 1984, EDIBLE AROIDS PREFAC
[8]  
CREIGHTON TE, 1984, PROTEINS STRUCTURES, P238
[9]   SELECTIVE CLEAVAGE OF SINGLE TRYPTOPHANYL PEPTIDE-BOND IN HORSE HEART CYTOCHROME C [J].
FONTANA, A ;
VITA, C ;
TONIOLO, C .
FEBS LETTERS, 1973, 32 (01) :139-142
[10]  
GROSS E, 1967, METHOD ENZYMOL, V11, P238