2 DIFFERENT ENZYMES ARE PRIMARILY RESPONSIBLE FOR RETINOIC ACID SYNTHESIS IN RABBIT LIVER CYTOSOL

被引：38

作者：

HUANG, DY

ICHIKAWA, Y

机构：

[1] Department of Biochemistry, Kagawa Medical School, Mikicho, Kita-gun

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.2803

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Retinoic acid biosynthesis in rabbit liver wets catalyzed by cytosolic NAD(+)-dependent dehydrogenase and oxygen-dependent oxidase, with;In activity ratio of 59% and 41% in the presence of 2 mM dithiothreitol under aerobic conditions. The two enzymes could be well separated by fractionation involving ammonium sulfate precipitation. Purification of the enzymes indicated that the oxygen-dependent enzyme was a flavoenzyme, retinal oxidase (EC 1.2.3.11), composed of two 135 kDa subunits; and the NAD(+)-dependent enzyme was a basic pI retinal dehydrogenase composed of four 55-kDa subunits. A high concentration (1-2 mM) of DTT was required to stabilize the activity of retinal dehydrogenase during the purification procedures and storage, but inhibited the activity of retinal oxidase by 13-38%. The physiological roles of the two retinoic acid synthases in liver cytosol were discussed. (C) 1994 Academic Press, Inc.

引用

页码：1278 / 1283

页数：6

共 23 条

[1] ENZYMATIC OXIDATION OF ALL-TRANS RETINAL TO RETINOIC ACID IN RAT-TISSUES [J].