A H-1-NMR NOE STUDY ON CO(II) STELLACYANIN - SOME CLUES ABOUT THE STRUCTURE OF THE METAL SITE

被引：30

作者：

VILA, AJ

机构：

[1] Cátedra de Biofísica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 2000 Rosario

来源：

FEBS LETTERS | 1994年 / 355卷 / 01期

关键词：

STELLACYANIN; BLUE COPPER PROTEIN; NMR; NOE; CO(II); METAL SUBSTITUTION;

D O I：

10.1016/0014-5793(94)01141-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The H-1 NMR spectrum of Co(II) stellacyanin is reported, in which four signals not previously observed have been detected. NOE experiments were performed to assign the hyperfine shifted signals corresponding to a Cys and two His residues. Both His residues are solvent-accessible and are shown to bind the metal ion through their N delta 1 atoms. The beta-CH2 Cys proton shifts indicate the presence of a strong axial ligand.

引用

页码：15 / 18

页数：4

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