INVOLVEMENT OF ANTIPAIN-SENSITIVE PROTEASE ACTIVITY IN SUPPRESSION OF UV-MUTAGENICITY BY HUMAN INTERFERON-ALPHA

被引：16

作者：

ISOGAI, E ^{[1
]}

SUZUKI, N ^{[1
]}

机构：

[1] CHIBA UNIV, SCH MED, DEPT BIOCHEM, CHUO KU, CHIBA 260, JAPAN

来源：

MUTATION RESEARCH LETTERS | 1994年 / 325卷 / 2-3期

关键词：

HUMAN CELL; MUTATION; INTERFERON-ALPHA; PROTEASE; ANTIPAIN;

D O I：

10.1016/0165-7992(94)90005-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To study the relationship between the transient elevation of protease activity and hypomutability observed in hypermutable human RSa cells pretreated with human interferon (HuIFN)-alpha and then irradiated with far-ultraviolet light (UV), protease inhibitors capable of specifically inhibiting the activity were investigated. Of ten inhibitors tested, antipain showed the greatest inhibitory effect. Antipain also prevented the suppression of UV-mutagenicity by HuIFN-alpha in RSa and xeroderma pigmentosum-derived fibroblast cells, as shown by culturing cells in medium containing antipain immediately after UV exposure and evaluating the generation of clones resistant to ouabain- or 6-thioguanine-mediated cytotoxicity. Thus, an antipain-sensitive protease may be involved in the hypomutability induced by HuIFN-alpha.

引用

页码：81 / 85

页数：5

共 20 条

[1] IDENTIFICATION AND EXPRESSION OF THE CDNA OF KIN17, A ZINC-FINGER GENE LOCATED ON MOUSE CHROMOSOME-2, ENCODING A NEW DNA-BINDING PROTEIN [J].

ANGULO, JF ;

ROUER, E ;

MAZIN, A ;

MATTEI, MG ;

TISSIER, A ;

HORELLOU, P ;

BENAROUS, R ;

DEVORET, R .

NUCLEIC ACIDS RESEARCH, 1991, 19 (19) :5117-5123

[2]

BARRETT AJ, 1986, RES MONOG CELL TISSU, V12, P515

[3]

BOOTHMAN DA, 1991, CANCER RES, V51, P5587

[4] IDENTIFICATION AND ISOLATION OF THE GENE ENCODING THE SMALL SUBUNIT OF RIBONUCLEOTIDE REDUCTASE FROM SACCHAROMYCES-CEREVISIAE - DNA DAMAGE-INDUCIBLE GENE REQUIRED FOR MITOTIC VIABILITY [J].

ELLEDGE, SJ ;

DAVIS, RW .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2783-2793

[5] EFFECTS OF A TUMOR PROMOTER AND AN ANTI-PROMOTER ON SPONTANEOUS AND UV-INDUCED 6-THIOGUANINE-RESISTANT MUTATIONS AND SISTER-CHROMATID EXCHANGES IN V79 CHINESE-HAMSTER CELLS [J].

FUJIWARA, Y ;

KANO, Y ;

TATSUMI, M ;

PAUL, P .

MUTATION RESEARCH, 1980, 71 (02) :243-251

[6] CONCEPTS AND MODELS FOR DNA-REPAIR - FROM ESCHERICHIA-COLI TO MAMMALIAN-CELLS [J].

HANAWALT, PC .

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 1989, 14 :90-98

[7] IDENTIFICATION OF THE GENE FOR THE YEAST RIBONUCLEOTIDE REDUCTASE SMALL SUBUNIT AND ITS INDUCIBILITY BY METHYL METHANESULFONATE [J].

HURD, HK ;

ROBERTS, CW ;

ROBERTS, JW .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (10) :3673-3677

[8] PARTIAL PURIFICATION AND PROPERTIES OF UROKINASE INHIBITOR FROM HUMAN PLACENTA [J].

KAWANO, T ;

MORIMOTO, K ;

UEMURA, Y .

JOURNAL OF BIOCHEMISTRY, 1970, 67 (03) :333-&

[9] WORKSHOP ON DNA-REPAIR [J].

LEHMANN, AR ;

HOEIJMAKERS, JHJ ;

VANZEELAND, AA ;

BACKENDORF, CMP ;

BRIDGES, BA ;

COLLINS, A ;

FUCHS, RPD ;

MARGISON, GP ;

MONTESANO, R ;

MOUSTACCHI, E ;

NATARAJAN, AT ;

RADMAN, M ;

SARASIN, A ;

SEEBERG, E ;

SMITH, CA ;

STEFANINI, M ;

THOMPSON, LH ;

VANDERSCHANS, GP ;

WEBER, CA ;

ZDZIENICKA, MZ .

MUTATION RESEARCH, 1992, 273 (01) :1-28

[10] HELA-CELLS LACK RECA GENE [J].

MISHRA, NC .

FEBS LETTERS, 1982, 137 (02) :175-177

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