COUPLING OF ESCHERICHIA-COLI HNS MESSENGER-RNA LEVELS TO DNA-SYNTHESIS BY AUTOREGULATION - IMPLICATIONS FOR GROWTH-PHASE CONTROL

被引:55
作者
FREE, A [1 ]
DORMAN, CJ [1 ]
机构
[1] UNIV DUBLIN TRINITY COLL, MOYNE INST PREVENT MED, DEPT MICROBIOL, DUBLIN 2, IRELAND
基金
英国惠康基金;
关键词
D O I
10.1111/j.1365-2958.1995.mmi_18010101.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The H-NS protein of enteric bacteria is one of the major proteins of the bacterial nucleoid and seems to play an important role in nucleoid structure. Transcription of the hns gene encoding the H-NS protein appears to be negatively regulated by H-NS itself both in vitro and in vivo. We have examined the role of this mode of regulation in wild-type cells in vivo. We find that hns transcription is down-regulated when DNA synthesis is blocked in growing cells, in a manner that is dependent upon continuing H-NS protein synthesis. These data suggest that hns autoregulation selves to match de novo H-NS synthesis to the demands of DNA synthesis and may maintain a relatively constant H-NS:DNA ratio. It has previously been suggested that hns transcription is activated as cells enter stationary phase, which would require a complete relaxation of autoregulatory control given that DNA synthesis decreases at this time. However, we show here that levels of hns mRNA in fact decline at the onset of stationary phase in a manner fully consistent with the autoregulation model. We also fail to detect any significant accumulation of the H-NS protein in stationary phase.
引用
收藏
页码:101 / 113
页数:13
相关论文
共 53 条
[1]   EXPRESSION OF THE GENES-CODING FOR THE ESCHERICHIA-COLI INTEGRATION HOST FACTOR ARE CONTROLLED BY GROWTH-PHASE, RPOS, PPGPP AND BY AUTOREGULATION [J].
AVIV, M ;
GILADI, H ;
SCHREIBER, G ;
OPPENHEIM, AB ;
GLASER, G .
MOLECULAR MICROBIOLOGY, 1994, 14 (05) :1021-1031
[2]   DRAMATIC CHANGES IN FIS LEVELS UPON NUTRIENT UPSHIFT IN ESCHERICHIA-COLI [J].
BALL, CA ;
OSUNA, R ;
FERGUSON, KC ;
JOHNSON, RC .
JOURNAL OF BACTERIOLOGY, 1992, 174 (24) :8043-8056
[3]   IDENTIFICATION OF 2 NEW GENETICALLY ACTIVE REGIONS ASSOCIATED WITH THE OSMZ LOCUS OF ESCHERICHIA-COLI - ROLE IN REGULATION OF PROU EXPRESSION AND MUTAGENIC EFFECT AT CYA, THE STRUCTURAL GENE FOR ADENYLATE-CYCLASE [J].
BARR, GC ;
BHRIAIN, NN ;
DORMAN, CJ .
JOURNAL OF BACTERIOLOGY, 1992, 174 (03) :998-1006
[4]   MUTATIONS IN BGLY, THE STRUCTURAL GENE FOR THE DNA-BINDING PROTEIN H1, AFFECT EXPRESSION OF SEVERAL ESCHERICHIA-COLI GENES [J].
BERTIN, P ;
LEJEUNE, P ;
LAURENTWINTER, C ;
DANCHIN, A .
BIOCHIMIE, 1990, 72 (12) :889-891
[5]   NUCLEOTIDE-SEQUENCE OF THE ESCHERICHIA-COLI THYMIDINE KINASE GENE PROVIDES EVIDENCE FOR CONSERVATION OF FUNCTIONAL DOMAINS AND QUATERNARY STRUCTURE [J].
BLACK, ME ;
HRUBY, DE .
MOLECULAR MICROBIOLOGY, 1991, 5 (02) :373-379
[6]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[7]   INTERACTION OF THE MAIN COLD SHOCK PROTEIN CS7.4 (CSPA) OF ESCHERICHIA-COLI WITH THE PROMOTER REGION OF HNS [J].
BRANDI, A ;
PON, CL ;
GUALERZI, CO .
BIOCHIMIE, 1994, 76 (10-11) :1090-1098
[8]   TRANSPOSITION AND FUSION OF LAC GENES TO SELECTED PROMOTERS IN ESCHERICHIA-COLI USING BACTERIOPHAGE-LAMBDA AND BACTERIOPHAGE-MU [J].
CASADABAN, MJ .
JOURNAL OF MOLECULAR BIOLOGY, 1976, 104 (03) :541-555
[9]   ACTIVITY OF A PLASMID-BORNE LEU-500 PROMOTER DEPENDS ON THE TRANSCRIPTION AND TRANSLATION OF AN ADJACENT GENE [J].
CHEN, DR ;
BOWATER, R ;
DORMAN, CJ ;
LILLEY, DMJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (18) :8784-8788
[10]   DNAB ANALOG SPECIFIED BY BACTERIOPHAGE P1 [J].
DARI, R ;
JAFFEBRACHET, A ;
TOUATISCHWARTZ, D ;
YARMOLINSKY, MB .
JOURNAL OF MOLECULAR BIOLOGY, 1975, 94 (03) :341-366