COLLAGENASE ACTIVITY IN CULTURES OF RAT PROSTATE CARCINOMA

被引：24

作者：

HUANG, CC ^{[1
]}

WU, CH ^{[1
]}

ABRAMSON, M ^{[1
]}

机构：

[1] COLUMBIA UNIV COLL PHYS & SURG, CTR CANC, INST CANC RES, NEW YORK, NY 10032 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 570卷 / 01期

关键词：

(Rat prostate); Carcinoma; Collagenase; Tissue invasion;

D O I：

10.1016/0005-2744(79)90209-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A specific collagenase (EC 3.4.24.3) has been found and purified from serum-free culture medium of 11095 epidermoid carcinoma of rat prostate. The molecular weight of this collagenase was estimated at 71 000 and the pH optimum was approx. 7. At 26°C, the collagenase cleaved collagen at a site 3/4 the length from the N-terminus. At 37°C, this collagenase degraded collagen to smaller peptides. The enzyme activity was inhibited by serum, cysteine and EDTA, but not by protease inhibitors. The presence of collagenase in rat tumor tissue suggests that this enzyme might play a significant role in tissue invasion by cancer cells. © 1979.

引用

页码：149 / 156

页数：8

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