PREPARATION AND HANDLING OF AEQUORIN SOLUTIONS FOR THE MEASUREMENT OF CELLULAR CA-2+

被引：52

作者：

SHIMOMURA, O

机构：

[1] Marine Biological Laboratory, Woods Hole, MA

来源：

CELL CALCIUM | 1991年 / 12卷 / 09期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0143-4160(91)90060-R

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Main characteristics of the various types of aequorin presently available for measuring cellular Ca2+, i.e. heterogeneous aequorin, isoaequorins, recombinant aequorin, fluorescein-labeled aequorin and semi-synthetic aequorins, are summarized. Basic techniques of preparing and handling the solutions of those aequorins for measuring Ca2+, including such techniques as concentrating aequorin solutions, freeze-drying, changing buffer composition, and the regeneration of active aequorin, are described.

引用

页码：635 / 643

页数：9

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