A SIMPLE METHOD FOR DIRECT CLONING CDNA SEQUENCE THAT FLANKS A REGION OF KNOWN SEQUENCE FROM TOTAL RNA BY APPLYING THE INVERSE POLYMERASE CHAIN-REACTION

被引：36

作者：

HUANG, SH

HU, YY

WU, CH

HOLCENBERG, J

机构：

[1] Division of Hematology/Oncology, Children Hospital of Los Angeles, Los Angeles

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 07期

关键词：

D O I：

10.1093/nar/18.7.1922

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：1922 / 1922

页数：1

共 7 条

[1]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[2]

HUANG SH, 1989, J BIOL CHEM, V264, P14762

[3]

MANIATIS T, 1982, MOL CLONING, P147

[4]

OCHMAN H, 1988, GENETICS, V120, P621

[5]

PROMEGA, 1989, PROTOCOLS APPLICATIO, P167

[6] PRIMER-DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA-POLYMERASE [J].

SAIKI, RK ;

GELFAND, DH ;

STOFFEL, S ;

SCHARF, SJ ;

HIGUCHI, R ;

HORN, GT ;

MULLIS, KB ;

ERLICH, HA .

SCIENCE, 1988, 239 (4839) :487-491

[7] A PROCEDURE FOR INVITRO AMPLIFICATION OF DNA SEGMENTS THAT LIE OUTSIDE THE BOUNDARIES OF KNOWN SEQUENCES [J].

TRIGLIA, T ;

PETERSON, MG ;

KEMP, DJ .

NUCLEIC ACIDS RESEARCH, 1988, 16 (16) :8186-8186

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