UPTAKE OF GLUTAMATE AND CYSTINE IN C-6 GLIOMA-CELLS AND IN CULTURED ASTROCYTES

被引：164

作者：

CHO, Y ^{[1
]}

BANNAI, S ^{[1
]}

机构：

[1] TSUKUBA UNIV,SCH MED,DEPT BIOCHEM,TSUKUBA,IBARAKI 305,JAPAN

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 55卷 / 06期

关键词：

Astrocytes; Cystine; C‐6 glioma cells; Glutamate transport; Glutathione;

D O I：

10.1111/j.1471-4159.1990.tb05800.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: The uptake of glutamate in rat glioma C‐6 cells and cultured astrocytes derived from rat cerebral hemispheres was found to be mediated by a Na+‐dependent and a Na+‐independent system. The Na+‐dependent system was inhibited by aspartate and was consistent with the commonly occurring system designated system X−ag. The Na+‐independent system was inhibited by cystine and was consistent with system x−c described in various types of cells in the periphery. It was also found that quisqualate selectively and competitively interfered with the Na+‐independent glutamate uptake. In C‐6 cells, the glutamate uptake via systems X−ag and x−c accounted for approximately 35% and 55% of the total uptake, respectively, at 0.05 mM glutamate. In cultured astrocytes, the glutamate uptake via system X−ag was very potent, whereas the uptake via system x−c was relatively weak and its contribution to the total uptake of glutamate seemed almost negligible. However, in both C‐6 cells and astrocytes, system x−c was necessary for the uptake of cystine, another substrate of system x−c. Cystine in the culture medium was an essential precursor of glutathione, and the inhibition of the cystine uptake by excess glutamate as a competitor led to a severe deficiency in glutathione, followed by cell degeneration. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：2091 / 2097

页数：7

共 39 条

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