OPTIMIZED DETERMINATION OF MALONDIALDEHYDE IN PLASMA-LIPID EXTRACTS USING 1,3-DIETHYL-2-THIOBARBITURIC ACID - INFLUENCE OF DETECTION METHOD AND RELATIONS WITH LIPIDS AND FATTY-ACIDS IN PLASMA FROM HEALTHY-ADULTS

被引：27

作者：

HOVING, EB ^{[1
]}

LAING, C ^{[1
]}

RUTGERS, HM ^{[1
]}

TEGGELER, M ^{[1
]}

VANDOORMAAL, JJ ^{[1
]}

MUSKIET, FAJ ^{[1
]}

机构：

[1] UNIV GRONINGEN,FAC MED,DEPT INTERNAL MED,DIV CLIN ENDOCRINOL,9700 AB GRONINGEN,NETHERLANDS

来源：

CLINICA CHIMICA ACTA | 1992年 / 208卷 / 1-2期

关键词：

PLASMA MALONDIALDEHYDE; SPECTROPHOTOMETRY; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; PLASMA LIPIDS; REFERENCE VALUES; LIPID PEROXIDATION;

D O I：

10.1016/0009-8981(92)90022-I

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

We investigated the influence of different concentrations of Fe3+, phosphoric acid, butylated hydroxytoluene and glutathione on the production of the malondialdehyde-1,3-diethyl-2-thiobarbituric acid adduct in plasma lipid extracts. Following organic solvent extraction the stable product was analyzed by spectrophotometry (537 nm), fluorometry (547 nm) and high-performance liquid chromatography with fluorometric detection. Using optimized reaction conditions there was good agreement between the three methods, with slightly higher values for the spectrophotometric method. Plasma total lipid malondialdehyde reference values for 24 healthy adults amounted to 1.30 +/- 0.23-mu-mol/l (spectrophotometric method) and 1.11 +/- 0.31-mu-mol/l (fluorometric method). Plasma lipid malondialdehyde concentrations correlated significantly with plasma triglycerides (r = 0.527), total cholesterol (r = 0.612) and total fatty acids (r = 0.810) and with the total number of double bonds present in plasma fatty acids with three or more double bonds (r = 0.923).

引用

页码：63 / 76

页数：14

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