IDENTIFICATION OF A C-KI-RAS ONCOGENE IN A NEOPLASM ISOLATED FROM WINTER FLOUNDER

被引：21

作者：

MCMAHON, G ^{[1
]}

HUBER, LJ ^{[1
]}

STEGEMAN, JJ ^{[1
]}

WOGAN, GN ^{[1
]}

机构：

[1] WOODS HOLE OCEANOG INST,DEPT BIOL,WOODS HOLE,MA 02543

来源：

MARINE ENVIRONMENTAL RESEARCH | 1988年 / 24卷 / 1-4期

关键词：

BIOLOGICAL MATERIALS - Chemical Analysis - MUTAGENS;

D O I：

10.1016/0141-1136(88)90333-9

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The purpose of this study was to determine whether transforming DNA sequences (oncogenes) could be identified in liver neoplasms isolated from feral fish and to study their relationship to their corresponding proto-oncogenes. The ultimate aim of this study is to characterize novel mutations in oncogenes derived from these hepatomas to correlate these genetic changes with chemical exposure history. DNA was prepared from transformed foci and analyzed by Southern blot hybridization to viral DNA probes specific for c-Ki-ras and c-Ha-ras DNA sequences. A c-Ki-ras oncogene was identified in a transformant derived from one of the two tumors assayed. Comparison of c-Ki-ras DNA sequences of tumor and tumor-derived transformants indicate that the activated oncogene in the transformant is of flounder origin. We are currently analyzing the flounder oncogene for activating point mutations by primer-directed enzymatic amplification and direct sequence analysis.

引用

页码：345 / 350

页数：6

共 10 条

[1] ONCOGENES AND HUMAN CANCER - CAUSE OR CONSEQUENCE [J].