ILVEDA OPERON OF ESCHERICHIA-COLI-K12 ENCODES ONLY ONE VALINE-ALPHA-KETOGLUTARATE TRANSAMINASE-ACTIVITY

被引：9

作者：

ADAMS, CW

LAWTHER, RP

HATFIELD, GW

机构：

[1] Department of Microbiology College, Medicine University of California, Irvine, Irvine

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 89卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0006-291X(79)90679-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transaminase B of E. coli K12 was purified to apparent homogeneity as measured by SDS acrylamide gel electrophoresis, immunoelectrophoresis, and amino terminal sequence analysis. The valine- and isoleucine-α-ketoglutarate dependent transaminase activities of pure enzyme as well as crude extracts were characterized by immunologic and kinetic methods. The data disprove the existence of a separate valine-α-ketoglutarate transaminase within the ilvEDA operon. © 1979.

引用

页码：650 / 658

页数：9

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