SYNTHESIS OF BACKBONE DEUTERIUM LABELED [R(CGCGAA-UUCGCG)]2 AND HPLC PURIFICATION OF SYNTHETIC RNA

被引:12
作者
KHARE, D [1 ]
ORBAN, J [1 ]
机构
[1] UNIV MARYLAND,MARYLAND BIOTECHNOL INST,CTR ADV RES BIOTECHNOL,9600 GUDELSKY DR,ROCKVILLE,MD 20850
关键词
D O I
10.1093/nar/20.19.5131
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The chemical synthesis of backbone deuterium labelled [r(CGCGAAU*U*CGCG)]2 (U* = [5'-H-2]U) is described. An efficient purification procedure was developed using a polymeric reverse phase (PRP) HPLC column at 60-degrees-C. This procedure provided pure RNA dodecamer in the multi-milligram quantities (39% overall yield) necessary for dynamics studies using solid-state deuterium NMR. The purification method has been effectively applied to other RNA sequences and will assist biophysical studies which require relatively large quantities of RNA oligomers.
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页码:5131 / 5136
页数:6
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