MORPHOLOGICAL, BIOCHEMICAL AND MOLECULAR CHARACTERISTICS OF METARHIZIUM-ANISOPLIAE AND M-FLAVOVIRIDE

被引：58

作者：

BRIDGE, PD ^{[1
]}

WILLIAMS, MAJ ^{[1
]}

PRIOR, C ^{[1
]}

PATERSON, RRM ^{[1
]}

机构：

[1] INT INST BIOL CONTROL,ASCOT SL5 7TA,BERKS,ENGLAND

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1993年 / 139卷

关键词：

D O I：

10.1099/00221287-139-6-1163

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Twenty-four isolates of Metarhizium anisopliae and M. flavoviride, screened for virulence to desert locust (Schistocerca gregaria), were characterized by conidial morphology, API ZYM, isoenzyme electrophoresis and RFLP analysis of HaeIII digests on agarose minigels. Conidial morphology showed considerable overlap in measurements between the two species. However the API ZYM method showed that all isolates of M. anisopliae differed from those of M. flavoviride by possessing alpha-fucosidase activity. Isoenzyme electrophoresis clearly distinguished the two species by catalase and acid phosphatase banding patterns. M. anisopliae had a much simpler RFLP banding pattern than M. flavoviride, which showed > 20 bands, but individual isolates of both species had unique patterns. The methods used could all contribute to a unique 'fingerprint' for isolates of both species including M. flavoviride IMI 330189, which has been under intensive study for biopesticide development against locusts and grasshoppers.

引用

页码：1163 / 1169

页数：7

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