AUTOMATED-DETERMINATION OF HYPOXANTHINE AND XANTHINE IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COLUMN-SWITCHING

被引：13

作者：

SUMI, S

KIDOUCHI, K

OHBA, S

WADA, Y

机构：

[1] NAGOYA CITY HIGASHI GEN HOSP,DEPT PEDIAT,CHIKUSA KU,NAGOYA,AICHI 464,JAPAN

[2] NAGOYA CITY UNIV,SCH MED,DEPT PEDIAT,MIZUHO KU,NAGOYA,AICHI 466,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 670卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00179-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a high-performance liquid chromatographic method with column switching for urinary hypoxanthine and xanthine. Analyses were carried out with both a reversed-phase column and an anion-exchange column connected by a column switch and controlled automatically by a computerized system controller. The relationships between standard concentrations and peak heights were linear in a concentration range of 1 to 1000 nmol/ml. The recovery of hypoxanthine added to urine was 101.1%, and that of xanthine was 98.1%. With our method urinary hypoxanthine and xanthine can be measured accurately without any sample preparation other than filtration.

引用

页码：376 / 378

页数：3

共 6 条

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