BIOSYNTHESIS OF TRITERPENOID SAPOGENOLS IN SOYBEAN AND ALFALFA SEEDLINGS

被引:9
作者
PERI, I
MOR, U
HEFTMANN, E
BONDI, A
TENCER, Y
机构
[1] USDA SEA,WESTERN REG RES CTR,BERKELEY,CA 94710
[2] HEBREW UNIV JERUSALEM,FAC AGR,REHOVOT,ISRAEL
关键词
biosynthesis; Glycine max; Leguminosae; Medicago sativa; triterpenoid sapogenols;
D O I
10.1016/0031-9422(79)80181-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
By incubation of germinating soybeans with mevalonate-[2-14C] (MVA), radioactivity was incorporated into four sapogenols which were identified by TLC. Unequivocal evidence for the identity of three of the four sapogenols was provided by co-crystallization to constant specific radioactivity. The partition of incorporated radioactivity into lipid- and water-soluble fractions and the pattern of radioactivity of individual sapogenols varied with the mode of administering labeled substrates to soybean seedlings, such as incubation of germinating soybeans with MVA-[2-14C], immersion of roots into MVA-[2-14C] or foliar application of squalene-[14C]. When alfalfa seedlings were incubated with MVA-[2-14C], about two-thirds of the radioactivity incorporated into the sapogenols was associated with medicagenic acid. © 1979.
引用
收藏
页码:1671 / 1674
页数:4
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