INVITRO MODEL FOR THE STUDY OF COLLAGEN DEGRADATION DURING ACUTE-INFLAMMATION

被引:15
作者
ETHERINGTON, DJ
SILVER, IA
GIBBONS, R
机构
[1] UNIV BRISTOL,SCH MED,DEPT PATHOL,BRISTOL BS8 1TD,AVON,ENGLAND
[2] UNIV BRISTOL,SCH MED,DEPT MED,BRISTOL BS8 1TD,AVON,ENGLAND
关键词
D O I
10.1016/0024-3205(79)90609-X
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Collagen sponges, labelled with fluorescein, were implanted under the back skin of sensitized rats. These elicited an acute inflammatory reaction with cellular invasion of the sponge and the development of a fibrous capsule at the periphery. After 4 days each sponge, together with the fibrous capsule, was excised and placed into tissue culture. Degradation of the collagen sponge by the invading cells was monitored from the release of soluble fluorescein peptides into the medium. The addition of foetal calf serum caused inhibition above 5% (v/v). Inhibitors of collagenase and neutral proteinases blocked the release of fluorescein peptides. Collagenolysis was also abolished or retarded by inhibitors of lysosomal cathepsins. The anti-inflammatory drug, dexamethasone, blocked all collagenolytic activity whereas indomethacin was without effect. The ex vivo model offers the possibility for following the activity of the invading phagocytic cells and for examining the enzymatic mechanisms involved in collagenolysis using appropriate perturbation techniques. © 1979.
引用
收藏
页码:1885 / 1891
页数:7
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