EFFICIENT TRANSFORMATION OF BACILLUS-THURINGIENSIS REQUIRES NONMETHYLATED PLASMID DNA

被引：66

作者：

MACALUSO, A ^{[1
]}

METTUS, AM ^{[1
]}

机构：

[1] ECOGEN INC,LANGHORNE,PA 19047

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 03期

关键词：

D O I：

10.1128/jb.173.3.1353-1356.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The transformation efficiency of Bacillus thuringiensis depends upon the source of plasmid DNA. DNA isolated from B. thuringiensis, Bacillus megaterium, or a Dam- Dcm- Escherichia coli strain efficiently transformed several B. thuringiensis strains. B. thuringiensis strains were grouped according to which B. thuringiensis backgrounds were suitable sources of DNA for transformation of other B. thuringiensis strains, suggesting that B. thuringiensis strains differ in DNA modification and restriction. Efficient transformation allowed the demonstration of developmental regulation of cloned crystal protein genes in B. thuringiensis.

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页码：1353 / 1356

页数：4

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