A ONE-PLATE MICROBIOLOGICAL SCREENING-TEST FOR ANTIBIOTIC RESIDUE TESTING IN KIDNEY TISSUE AND MEAT - AN ALTERNATIVE TO THE EEC 4-PLATE METHOD

被引:42
作者
KOENENDIERICK, K
OKERMAN, L
DEZUTTER, L
DEGROODT, JM
VANHOOF, J
SREBRNIK, S
机构
[1] Ministry of Public Health, Institute of Hygiene and Epidemiology, Food Division, Brussels, B-1050
[2] University of Ghent, Faculty of Veterinary Medecine, Department of Veterinary Food Inspection, Ghent, B-9000
来源
FOOD ADDITIVES AND CONTAMINANTS | 1995年 / 12卷 / 01期
关键词
ANTIMICROBIALS; RESIDUE MONITORING; KIDNEY TISSUE; MEAT; INHIBITION TEST;
D O I
10.1080/02652039509374281
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A one-plate screening method is described for the microbiological detection of antibiotic residues by growth inhibition of Bacillus subtilis in agar medium, at pH 7, which contains trimethoprim for better detection of sulphonamides. beta-Glucuronidase is added to the samples to enable the detection of chloramphenicol residues. The sensitivity was determined for 16 antimicrobial substances frequently used in animal husbandry. A comparison was made with the sensitivity of the EEC four-plate test and existing maximum residue levels. The method has been used in Belgium for 5 years for the monitoring of antimicrobial residues in kidney tissue and meat of slaughter animals.
引用
收藏
页码:77 / 82
页数:6
相关论文
共 13 条
[1]  
Anonymous, Ministrieel besluit van 18 december 1973 tot bepaling van de laboratoriumtechnieken voor het opsporen van residuen van stoffen met een kiemgroeiremmende werking, Belgisch Staatsblad, (1974)
[2]  
Anonymous, Commission regulation (EEC) No. 675/92 of 18 March 1992 amending Annexes I and III to council regulation (EEC) No. 2377/90 laying down a community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin, Official Journal of the European Communities, L73, pp. 8-14, (1992)
[3]  
Anonymous, Commission regulation (EEC) No. 3093/92 of 27 October 1992 amending Annex III to council regulation (EEC) No. 2377/90 laying down a community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin, Official Journal of the European Communities, L311, pp. 18-19, (1992)
[4]  
Anonymous, Commission regulation (EEC) No. 2091/93 of 18 October 1993 amending Annex I, II, III and IV to council regulation (EEC) No. 2377/90 laying down a community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin, Official Journal of the European Communities, L264, pp. 1-4, (1993)
[5]  
Bogaerts R., Wolf F., A standardized method for the detection of residues of anti-bacterial substances in fresh meat, Fleischwirtschaft, 60, pp. 672-673, (1980)
[6]  
Brush P.J., Wood J.M.P., Shaw I.C., Preferential distribution of streptomycin in pig kidney cortex, Analyst, 115, (1990)
[7]  
De Zutter L., Koenen-Dierick K., Van Hoof J., Detection of antimicrobial residues in slaughtered animals: II. Sensitivity of some detection methods to different antibiotics, Vlaams Diergeneeskundig Tijdschrift, 54, pp. 445-454, (1985)
[8]  
Ebrecht A., Verbesserung des Hemmstofftestes durch Zusatz von Trimethoprim zum Nachweis von Sulfonamiden, Archiv für Lebensmittelhygiene, 33, pp. 109-115, (1982)
[9]  
Ellerbroek L., Zum microbiologischen Nachweis der Chinoloncarbonsäurederivate Enrofloxacin, Ciprofloxacin und Flumequin, Fleischwirtschaft, 77, pp. 187-189, (1991)
[10]  
Heinert H., Van Der Wall G., Bremher H., Lysozym-Ursache von unspezifischen Reaktionen im mikrobiologischen Hemmstofftest. Archiv für, Archiv für Lebensmittelhygiene, 27, pp. 41-80, (1976)