Up-regulation of narX, encoding a putative 'fused nitrate reductase' in anaerobic dormant Mycobacterium bovis BCG

被引：41

作者：

Hutter, B ^{[1
]}

Dick, T ^{[1
]}

机构：

[1] Inst Mol & Cell Biol, Singapore 117609, Singapore

来源：

FEMS MICROBIOLOGY LETTERS | 1999年 / 178卷 / 01期

关键词：

dormancy; anaerobiosis; gene expression; nitrate reductase;

D O I：

10.1016/S0378-1097(99)00348-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mycobacterium tuberculosis and its closely related but non-pathogenic relative M. bovis Bacille Calmette-Guerin (BCG) have the capability to adapt to anaerobiosis by shifting down from aerobic growth to a stale of non-replicating persistence or dormancy. Here, we report the results of a comparative Northern analysis of 23 genes identified in the tubercle bacillus genome project that might play a role in the energy metabolism under anaerobic conditions. The expression of a majority of the genes was found to be down-regulated in the dormant BCG Culture. However, the mRNA level for narX, a putative 'fused nitrate reductase' not found in other bacteria, was strongly up-regulated in anaerobic dormant bacilli. narX is the first transcriptionally induced gene in anaerobic dormant mycobacteria and might be a useful marker for monitoring the dormancy response in infected animals. (C) 1999 Published by Elsevier Science B.V. All rights reserved.

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页码：63 / 69

页数：7

相关论文

共 17 条

[1]

[Anonymous], 1992, Curr Opin Neurobiol, V2, P1, DOI 10.1016/0959-4388(92)90152-B

[2] Enzymes and associated electron transport systems that catalyse the respiratory reduction of nitrogen oxides and oxyanions [J].

Berks, BC ;

Ferguson, SJ ;

Moir, JWB ;

Richardson, DJ .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1995, 1232 (03) :97-173

[3] MOLYBDENUM COFACTOR NEGATIVE MUTANTS OF ESCHERICHIA-COLI USE CITRATE ANAEROBICALLY [J].

CLARK, DP .

FEMS MICROBIOLOGY LETTERS, 1990, 67 (03) :245-250

[4] Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence [J].

Cole, ST ;

Brosch, R ;

Parkhill, J ;

Garnier, T ;

Churcher, C ;

Harris, D ;

Gordon, SV ;

Eiglmeier, K ;

Gas, S ;

Barry, CE ;

Tekaia, F ;

Badcock, K ;

Basham, D ;

Brown, D ;

Chillingworth, T ;

Connor, R ;

Davies, R ;

Devlin, K ;

Feltwell, T ;

Gentles, S ;

Hamlin, N ;

Holroyd, S ;

Hornby, T ;

Jagels, K ;

Krogh, A ;

McLean, J ;

Moule, S ;

Murphy, L ;

Oliver, K ;

Osborne, J ;

Quail, MA ;

Rajandream, MA ;

Rogers, J ;

Rutter, S ;

Seeger, K ;

Skelton, J ;

Squares, R ;

Squares, S ;

Sulston, JE ;

Taylor, K ;

Whitehead, S ;

Barrell, BG .

NATURE, 1998, 393 (6685) :537-+

[5] Mycobacterial stationary phase induced by low oxygen tension:: Cell wall thickening and localization of the 16-kilodalton α-crystallin homology [J].

Cunningham, AF ;

Spreadbury, CL .

JOURNAL OF BACTERIOLOGY, 1998, 180 (04) :801-808

[6] A stationary-phase stress-response sigma factor from Mycobacterium tuberculosis [J].

DeMaio, J ;

Zhang, Y ;

Ko, C ;

Young, DB ;

Bishai, WR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (07) :2790-2794

[7] Transcription of two sigma 70 homologue genes, sigA and sigB, in stationary-phase Mycobacterium tuberculosis [J].

Hu, JM ;

Coates, ARM .

JOURNAL OF BACTERIOLOGY, 1999, 181 (02) :469-476

[8]

Hu YM, 1998, FEMS MICROBIOL LETT, V158, P139

[9] Transcription of the stationary-phase-associated hspX gene of Mycobacterium tuberculosis is inversely related to synthesis of the 16-kilodalton protein [J].

Hu, YM ;

Coates, ARM .

JOURNAL OF BACTERIOLOGY, 1999, 181 (05) :1380-1387

[10] Oxygen depletion-induced dormancy in Mycobacterium bovis BCG [J].

Lim, A ;

Eleuterio, M ;

Hutter, B ;

Murugasu-Oei, B ;

Dick, T .

JOURNAL OF BACTERIOLOGY, 1999, 181 (07) :2252-2256