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INDUCTION OF THE HIGH-AFFINITY NA+-DEPENDENT GLUTAMATE TRANSPORT-SYSTEM X(AG)- BY HYPERTONIC STRESS IN THE RENAL EPITHELIAL-CELL LINE NBL-1

被引：22
作者：
FERRERMARTINEZ, A
FELIPE, A
NICHOLSON, B
CASADO, J
PASTORANGLADA, M
MCGIVAN, J
机构：
[1] UNIV BRISTOL,SCH MED SCI,DEPT BIOCHEM,BRISTOL BS8 1TD,AVON,ENGLAND
[2] UNIV BARCELONA,DEPT BIOQUIM & FISIOL,UNITATA BIOQUIM & BIOL MOLEC,E-08028 BARCELONA,SPAIN
来源：
BIOCHEMICAL JOURNAL | 1995年 / 310卷
关键词：
D O I：
10.1042/bj3100689
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The high-affinity Na+-dependent glutamate transport system X(AG-) is induced (threefold increase in V-max with no change in K-m) by hypertonicity in the renal epithelial cell line NBL-1. This effect is dependent on protein synthesis and glycosylation and is accompanied by an increase in EAAC1 mRNA levels. Other Na+-dependent transport systems in this cell line do not respond to hypertonic stress. In contrast to recent findings [Ruiz-Montasell, Gomez-Angelats, Casado, Felipe, McGivan and Pastor-Anglada (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 9569-9573] showing that increased system A activity after hyperosmotic shock results from induction of a regulatory protein, this is the first demonstration that hypertonicity may increase the expression of the gene for an amino acid transport protein itself.
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收藏
页码：689 / 692
页数：4
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