BINDING-SITE ON SV40 DNA FOR A T-ANTIGEN - RELATED PROTEIN

被引：436

作者：

TJIAN, R

机构：

[1] Cold Spring Harbor Laboratory Cold Spring Harbor

来源：

CELL | 1978年 / 13卷 / 01期

关键词：

D O I：

10.1016/0092-8674(78)90147-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A protein closely related to SV40 T antigen was purified in a biologically active form from cells infected with the defective adenovirus-SV40 hybrid, Ad2+D2. This 107,000 dalton hybrid protein binds and protects a specific portion of SV40 DNA from digestion by pancreatic DNAase I. Hybridization, endonuclease cleavage and pyrimidine tract analysis of the protected fragments reveal that the D2 hybrid protein binds in a sequential manner to tandem recognition sites which lie within a sequence of 120 nucleotides at position 67 near the origin of SV40 replication. © 1978.

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页码：165 / 179

页数：15

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